Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10336] to ADRA1B - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ADRA1B antibody [EPR10336] - BSA and Azide free
    See all ADRA1B primary antibodies

  • Description

    Rabbit monoclonal [EPR10336] to ADRA1B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab245776 is the carrier-free version of ab169523. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab245776 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as alpha 1b Adrenergic Receptor

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)

    This data was developed using ab169523, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining predominantly on collecting duct of human kidney. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.

  • Western blot - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)
    Western blot - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)
    All lanes : Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/2000 dilution (Purified)

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Mouse stomach tissue lysate
    Lane 7 : Rat stomach tissue lysate
    Lane 8 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa



    This data was developed using ab169523, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)

    This data was developed using ab169523, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human ovarian carcinoma. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)

    This data was developed using ab169523, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat stomach. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)

    This data was developed using ab169523, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse stomach. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)

    This data was developed using ab169523, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human breast carcinoma. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.

  • Western blot - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)
    Western blot - Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)
    Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/1000 dilution (unpurified) + HeLa cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 57 kDa



    This data was developed using ab169523, the same antibody clone in a different buffer formulation.

  • Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)
    Anti-ADRA1B antibody [EPR10336] - BSA and Azide free (ab245776)

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