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Signal Transduction Signaling Pathway Lipid Signaling PLC

Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5951] to Phospholipase C beta 3/PLCB3
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951]
    See all Phospholipase C beta 3/PLCB3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5951] to Phospholipase C beta 3/PLCB3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    corresponding to Human Phospholipase C beta 3/PLCB3 aa 1150-1250.

  • Positive control

    • HCT 116, 293T, and A431 cell lysates; Human breast and colon tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Phospholipase C beta 3.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5951
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735)
    Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735)
    All lanes : Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735) at 1/1000 dilution

    Lane 1 : HCT 116 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 139 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735)

    ab124735 at 1/100 dilution staining Phospholipase C beta 3/PLCB3 in paraffin-embedded Human breast tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735)

    ab124735 at 1/100 dilution staining Phospholipase C beta 3/PLCB3 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735)
    Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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