Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794)
Key features and details
- Rabbit polyclonal to Phospholipase C beta 3/PLCB3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase C beta 3/PLCB3 antibody
See all Phospholipase C beta 3/PLCB3 primary antibodies -
Description
Rabbit polyclonal to Phospholipase C beta 3/PLCB3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Recombinant fragment within Human Phospholipase C beta 3/PLCB3 (internal sequence). The exact sequence is proprietary.
Database link: Q01970 -
Positive control
- WB: MCF7, MDA-MB-231, HEK-293T, A431, HeLa, PC-3, Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12 and Rat2 whole cell lysate.
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General notes
This product was previously labelled as Phospholipase C beta 3
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794)All lanes : Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 139 kDa5% SDS-PAGE
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Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794)All lanes : Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 139 kDa5% SDS-PAGE
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Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794)Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794) at 1/1000 dilution + PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 139 kDa5% SDS-PAGE
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Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794)All lanes : Anti-Phospholipase C beta 3/PLCB3 antibody (ab228794) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8D30 whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 7 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 139 kDa5% SDS-PAGE
