Anti-Phospholipase C beta 2/PLCB2 antibody (ab272919)
Key features and details
- Rabbit polyclonal to Phospholipase C beta 2/PLCB2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase C beta 2/PLCB2 antibody
See all Phospholipase C beta 2/PLCB2 primary antibodies -
Description
Rabbit polyclonal to Phospholipase C beta 2/PLCB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Phospholipase C beta 2/PLCB2 (C terminal). The exact sequence is proprietary.
Database link: Q00722 -
Positive control
- IHC-P: Human colon cancer and lung cancer tissue. WB: THP-1 and HL-60 whole cell extract. ICC/IF: THP-1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Phospholipase C beta 2/PLCB2 antibody (ab272919)All lanes : Anti-Phospholipase C beta 2/PLCB2 antibody (ab272919) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell extract
Lane 2 : THP-1 (Human monocytic leukemia cell line) whole cell extract
Lane 3 : HL-60 (Human promyelocytic leukemia cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 2/PLCB2 antibody (ab272919)Paraffin-embedded human colon cancer tissue stained for Phospholipase C beta 2/PLCB2 using ab272919 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C beta 2/PLCB2 antibody (ab272919)4% PFA-fixed THP-1 (Human monocytic leukemia cell line) cells stained for Phospholipase C beta 2/PLCB2 using ab272919 at 1/500 dilution in ICC/IF (green). Blue: DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 2/PLCB2 antibody (ab272919)Paraffin-embedded human lung cancer tissue stained for Phospholipase C beta 2/PLCB2 using ab272919 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.