Anti-SHP2 antibody [Y477] (ab32159)
![Anti-SHP2 antibody [Y477] (ab32159)](https://www.abcam.com/ps/products/32/ab32159/Images/ab32159-259115-anti-shp2-antibody-y477-western-blot.jpg "Anti-SHP2 antibody [Y477] (ab32159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y477] to SHP2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SHP2 antibody [Y477]
See all SHP2 primary antibodies -
Description
Rabbit monoclonal [Y477] to SHP2 -
Host species
Rabbit -
Specificity
ab32159 recognises SHP2. It is predicted to detect splice isoforms 2 and 3 based on sequence analysis. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human SHP2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q06124 -
Positive control
- WB: Wild-type HAP1 cell lysate. Jurkat and A431 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
Y477 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SHP2 antibody [Y477] (ab32159)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SHP2 knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 to 4: Merged signal (red and green). Green - ab32159 observed at 68 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32159 was shown to specifically react with SHP2 when SHP2 knockout samples were used. Wild-type and SHP2 knockout samples were subjected to SDS-PAGE. ab32159 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-SHP2 antibody [Y477] (ab32159)](https://www.abcam.com/ps/products/32/ab32159/Images/ab32159-44075-anti-shp2-antibody-y477-western-blot.jpg)
Western blot - Anti-SHP2 antibody [Y477] (ab32159)Anti-SHP2 antibody [Y477] (ab32159) at 1/1000 dilution + Jurkat cell lysate.
Predicted band size: 68 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
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![Anti-SHP2 antibody [Y477] (ab32159)](https://www.abcam.com/ps/products/32/ab32159/Images/ab32159-3-benefits-of-recombinant-antibodies.png)
Anti-SHP2 antibody [Y477] (ab32159)
