Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SHP2 antibody [EPR17829-9] (ab187040)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17829-9] to SHP2
Suitable for: IP, WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-SHP2 antibody [EPR17829-9]
See all SHP2 primary antibodies
Description

Rabbit monoclonal [EPR17829-9] to SHP2
Host species

Rabbit

Tested Applications & Species

Application	Species
IP	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, Jurkat, HEK-293 and NIH/3T whole cell lysates; mouse brain and heart lysates; rat brain lysate. IP: HeLa and NIH/3T3 whole cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17829-9
Isotype

IgG
Research areas

Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040)

All lanes : Anti-SHP2 antibody [EPR17829-9] (ab187040) at 1/5000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PTPN11 knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 68 kDa
Observed band size: 68 kDa

Lanes 1- 2: Merged signal (red and green). Green - ab187040 observed at 68 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab187040 was shown to react with SHP2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266450 (knockout cell lysate ab257618) was used. Wild-type HEK-293T and PTPN11 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab187040 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040)

SHP2 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab187040 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab187040 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: HeLa whole cell lysate 10 μg (Input).

Lane 2: ab187040 IP in HeLa whole cell lysate (+).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187040 in HeLa whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-SHP2 antibody [EPR17829-9] (ab187040)

All lanes : Anti-SHP2 antibody [EPR17829-9] (ab187040) at 1/5000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse heart tissue lysate
Lane 7 : Rat brain tissue lysate
Lane 8 : Rat heart tissue lysate

Lysates/proteins at 5 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 1 minute

Blocking and dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-SHP2 antibody [EPR17829-9] (ab187040)

SHP2 was immunoprecipitated from 1 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab187040 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab187040 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: NIH/3T3 whole cell lysate 10μg (Input).

Lane 2: ab187040 IP in NIH/3T3 whole cell lysate (+).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187040 in NIH/3T3 whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-SHP2 antibody [EPR17829-9] (ab187040)

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