Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)
![Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)](https://www.abcam.com/ps/products/225/ab225574/Images/ab225574-2-ab62322IP1.jpg "Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP508(2)Y] to SHP2 (phospho Y542) - BSA and Azide free
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free
See all SHP2 primary antibodies -
Description
Rabbit monoclonal [EP508(2)Y] to SHP2 (phospho Y542) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Treated NIH/3T3 cell lysate.
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General notes
ab225574 is the carrier-free version of ab62322.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP508(2)Y -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)
This ICC/IF data was generated using the same anti-SHP2 (phospho Y542) antibody clone [EP608(2)Y] in a different buffer formulation (cat# ab62322).
ab62322 (purified) at 1:30 dilution (2µg) immunoprecipitating SHP2 in NIH/3T3 treated with 50ng/ml PDGF for 40min whole cell lysate.
Lane 1 (input): NIH/3T3 (Mouse embryonic fibroblast) treated with 50ng/ml PDGF for 40min whole cell lysate 10µg
Lane 2 (+): ab62322 & NIH/3T3 treated with 50ng/ml PDGF for 40min whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab62322 in NIH/3T3 treated with 50ng/ml PDGF for 40min whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Immunocytochemistry/ Immunofluorescence - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)](https://www.abcam.com/ps/products/225/ab225574/Images/ab225574-286195-anti-shp2-phospho-y542-antibody-ep5082y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)This ICC/IF data was generated using the same anti-SHP2 (phospho Y542) antibody clone [EP608(2)Y] in a different buffer formulation (cat# ab62322).
Ab62322 staining SHP2 in THP-1 cells ( Human monocytic leukaemia cell line) by ICC/IF (immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with purified ab62322 at 8.8 μg/ml. Secondary antibody used was AlexaFluor®488 Goat anti-Rabbit (ab150077) at 2 μg/ml. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)( ab195889) used as counterstain at 2.5 μg/ml . DAPI was used as nuclear counterstain. Confocal image showing the expression was increased after treatment with TPA 200nM for 24h and PDGF 50ng/ml for 30min, the signal decreased after treatment with Lambda Protein Phosphatase 31℃ for 2h.
This image was generated using the unpurified version of the product.
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![Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)](https://www.abcam.com/ps/products/225/ab225574/Images/ab225574-3-benefits-of-recombinant-antibodies.png)
Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)
