Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP508(2)Y] to SHP2 (phospho Y542) - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free
    See all SHP2 primary antibodies

  • Description

    Rabbit monoclonal [EP508(2)Y] to SHP2 (phospho Y542) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Treated NIH/3T3 cell lysate.

  • General notes

    ab225574 is the carrier-free version of ab62322.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Images

  • Immunoprecipitation - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)
    Immunoprecipitation - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)

    This ICC/IF data was generated using the same anti-SHP2 (phospho Y542) antibody clone [EP608(2)Y] in a different buffer formulation (cat# ab62322).

    ab62322 (purified) at 1:30 dilution (2µg) immunoprecipitating SHP2 in NIH/3T3 treated with 50ng/ml PDGF for 40min whole cell lysate.
    Lane 1 (input): NIH/3T3 (Mouse embryonic fibroblast) treated with 50ng/ml PDGF for 40min whole cell lysate 10µg
    Lane 2 (+): ab62322 & NIH/3T3 treated with 50ng/ml PDGF for 40min whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab62322 in NIH/3T3 treated with 50ng/ml PDGF for 40min whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)
    Immunocytochemistry/ Immunofluorescence - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)

    This ICC/IF data was generated using the same anti-SHP2 (phospho Y542) antibody clone [EP608(2)Y] in a different buffer formulation (cat# ab62322).

    Ab62322 staining SHP2 in THP-1 cells ( Human monocytic leukaemia cell line) by ICC/IF (immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with purified ab62322 at 8.8 μg/ml. Secondary antibody used was AlexaFluor®488 Goat anti-Rabbit (ab150077) at 2 μg/ml. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)( ab195889) used as counterstain at 2.5 μg/ml . DAPI was used as nuclear counterstain. Confocal image showing the expression was increased after treatment with TPA 200nM for 24h and PDGF 50ng/ml for 30min, the signal decreased after treatment with Lambda Protein Phosphatase 31℃ for 2h.

    This image was generated using the unpurified version of the product. 

  • Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)
    Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] - BSA and Azide free (ab225574)

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