Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TCPTP antibody [EPR6712] - BSA and Azide free (ab248278)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6712] to PTP1B + TCPTP - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra), IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PTP1B + TCPTP antibody [EPR6712] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR6712] to PTP1B + TCPTP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), IPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HL60, NIH 3T3, MOLT4 and HepG2 whole cell lysate (ab7900); Mouse placenta and thymus lysates.

  • General notes

    ab248278 is the carrier-free version of ab129070.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TCPTP antibody [EPR6712] - BSA and Azide free (ab248278)
    Western blot - Anti-TCPTP antibody [EPR6712] - BSA and Azide free (ab248278)
    All lanes : Anti-TCPTP antibody [EPR6712] (ab129070) at 1/1000 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : NIH/3T3 cell lysate
    Lane 3 : Mouse placenta tissue lysate
    Lane 4 : Mouse thymus tissue lysate
    Lane 5 : MOLT4 cell lysate
    Lane 6 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 48 kDa
    Observed band size: 45-48 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab129070, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-TCPTP antibody [EPR6712] - BSA and Azide free (ab248278)
    Flow Cytometry - Anti-TCPTP antibody [EPR6712] - BSA and Azide free (ab248278)
    This data was developed using ab129070, the same antibody clone in a different buffer formulation.Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) cell line fixed with 4% paraformaldehyde and permeabilized with 90% methanol labeling TCPTP with ab129070 at 1:200 dilution (red). This is compared with a Rabbit monoclonal IgG – Isotype control (ab172730) (black) and a unalbelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1:2000 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-TCPTP antibody [EPR6712] - BSA and Azide free (ab248278)
    Immunoprecipitation - Anti-TCPTP antibody [EPR6712] - BSA and Azide free (ab248278)

    This data was developed using ab129070, the same antibody clone in a different buffer formulation.

    Lane 1 (input): MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate, 10µg

    Lane 2 (+): MOLT-4 whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129070 in MOLT-4 whole cell lysate.

     ab129070 immunoprecipitating TCPTP in MOLT-4 whole cell lysate. Capture antibody was used at a 1:20 dilution (0.6µg in 0.35mg lysates). For western blotting, primary antibody used was ab129070 at 1:1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-TCPTP antibody [EPR6712] - BSA and Azide free (ab248278)
    Anti-TCPTP antibody [EPR6712] - BSA and Azide free (ab248278)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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