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Anti-PTPN12 antibody [BL-5-2F8] (ab243864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BL-5-2F8] to PTPN12
  • Suitable for: WB, IP, ICC, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PTPN12 antibody [BL-5-2F8]
    See all PTPN12 primary antibodies

  • Description

    Rabbit monoclonal [BL-5-2F8] to PTPN12

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PTPN12 aa 499-780. The exact sequence is proprietary. NP_002826.2 and Gene ID 5782.
    Database link: Q05209

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.8
    Preservative: 0.09% Sodium azide
    Constituents: 98% Borate buffered saline, 0.1% BSA
  • Concentration information loading...

  • Purification notes

    Recombinant antibody was purified from cell culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    BL-5-2F8

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)
    Immunocytochemistry - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)
    Formaldehyde-fixed SW620 cells labeling PTPN12 using ab243864 at 1/100 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)
    Formalin-fixed, paraffin-embedded human non-small cell lung cancer tissue stained for PTPN12 using ab243864 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)
    Formalin-fixed, paraffin-embedded human Mammary gland tissue stained for PTPN12 using ab243864 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
  • Immunoprecipitation - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)
    Immunoprecipitation - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)
    PTPN12 was immunoprecipitated from 0.5 mg HeLa whole cell lysate with ab243864 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243864 at 1/1000 dilution.

    Lane 1: ab243864 IP in HeLa cell lysate.

    Lane 2: Contol IgG in HeLa cell lysate.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

  • Western blot - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)
    Western blot - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)

    Western blot analysis using ab243864 at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate (5 µg).

    Lane 2: HEK-293T whole cell lysate (5 µg).

    Lane3: Jurkat whole cell lysate (5 µg).

    Lane 4: MCF-7 whole cell lysate (5 µg).

    Lane 5: HepG2 whole cell lysate (5 µg).

    Lane 6: A549 whole cell lysate (5 µg).

    Lane 7: SW620 whole cell lysate (5 µg).

    Lane 8: SK whole cell lysate (5 µg).

    Lane 9: HCT 116 whole cell lysate (5 µg).

    a HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds

  • Anti-PTPN12 antibody [BL-5-2F8] (ab243864)
    Anti-PTPN12 antibody [BL-5-2F8] (ab243864)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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