Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PTPN12 antibody [BL-5-2F8] (ab243864)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-5-2F8] to PTPN12
Suitable for: WB, IP, ICC, IHC-P
Reacts with: Human

Product name

Anti-PTPN12 antibody [BL-5-2F8]
See all PTPN12 primary antibodies
Description

Rabbit monoclonal [BL-5-2F8] to PTPN12
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human PTPN12 aa 499-780. The exact sequence is proprietary. NP_002826.2 and Gene ID 5782.
Database link: Q05209
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-5-2F8
Isotype

IgG
Research areas

Immunocytochemistry - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)

Formaldehyde-fixed SW620 cells labeling PTPN12 using ab243864 at 1/100 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)

Formalin-fixed, paraffin-embedded human non-small cell lung cancer tissue stained for PTPN12 using ab243864 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)

Formalin-fixed, paraffin-embedded human Mammary gland tissue stained for PTPN12 using ab243864 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunoprecipitation - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)

PTPN12 was immunoprecipitated from 0.5 mg HeLa whole cell lysate with ab243864 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243864 at 1/1000 dilution.
Lane 1: ab243864 IP in HeLa cell lysate.

Lane 2: Contol IgG in HeLa cell lysate.

Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-PTPN12 antibody [BL-5-2F8] (ab243864)

Western blot analysis using ab243864 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (5 µg).
Lane 2: HEK-293T whole cell lysate (5 µg).
Lane3: Jurkat whole cell lysate (5 µg).
Lane 4: MCF-7 whole cell lysate (5 µg).
Lane 5: HepG2 whole cell lysate (5 µg).
Lane 6: A549 whole cell lysate (5 µg).
Lane 7: SW620 whole cell lysate (5 µg).
Lane 8: SK whole cell lysate (5 µg).
Lane 9: HCT 116 whole cell lysate (5 µg).
a HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
Anti-PTPN12 antibody [BL-5-2F8] (ab243864)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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