Anti-PTPN12 antibody (ab76942)
Key features and details
- Rabbit polyclonal to PTPN12
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PTPN12 antibody
See all PTPN12 primary antibodies -
Description
Rabbit polyclonal to PTPN12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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Immunogen
Synthetic peptide corresponding to a region between residue 760 and 780 of human PTPN12 (NP_002826.2)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab76942 was affinity purified using an epitope specific to PTPN12 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody (ab76942)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and colon carcinoma (right) tissues labelling PTPN12 with ab76942 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-PTPN12 antibody (ab76942)All lanes : Anti-PTPN12 antibody (ab76942) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 88 kDa
Observed band size: 118 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunoprecipitation - Anti-PTPN12 antibody (ab76942)Lane 1: ab76942, at 1 µg/mg, staining PTPN12 in HeLa whole cell lysate by Western blot of immunoprecipitate (1 mg of lysate for IP, 20% of IP loaded). Immunoprecipitation was performed by using ab76942 at 3µg/mg of lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 3 seconds.
