Anti-PTPN12 antibody - N-terminal (ab154892)
Key features and details
- Rabbit polyclonal to PTPN12 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PTPN12 antibody - N-terminal
See all PTPN12 primary antibodies -
Description
Rabbit polyclonal to PTPN12 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
-
Immunogen
Recombinant fragment corresponding to a region within N terminal amino acids 1-207 of PTPN12 (Q05209)
-
Positive control
- Recombinant Human PTPN12 protein (ab132054) can be used as a positive control in WB. A549, HeLa or HepG2 lysate, HeLa cells, D54 xenograft, RT2 xenograft or Human ulcerative colitis tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PTPN12 antibody - N-terminal (ab154892)Anti-PTPN12 antibody - N-terminal (ab154892) at 1/5000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 88 kDa
5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody - N-terminal (ab154892)Immunohistochemical analysis of paraffin embedded Human D54 xenograft tissue labeling PTPN12 with ab154892 at a 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody - N-terminal (ab154892)Immunohistochemical analysis of paraffin embedded Human RT2 xenograft tissue labeling PTPN12 with ab154892 at a 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody - N-terminal (ab154892)Immunohistochemical analysis of paraffin embedded Human ulcerative colitis tissue labeling PTPN12 with ab154892 at a 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PTPN12 antibody - N-terminal (ab154892)Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PTPN12 with ab154892 at a 1/200 dilution (top image), and costained with Hoescht 33342 (bottom image)
