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Signal Transduction Protein Phosphorylation Tyrosine Phosphatases

Anti-TCPTP antibody [T25-AF3B7] (ab85330)

Anti-TCPTP antibody [T25-AF3B7] (ab85330)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [T25-AF3B7] to TCPTP
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-TCPTP antibody [T25-AF3B7]
    See all TCPTP primary antibodies
  • Description

    Mouse monoclonal [T25-AF3B7] to TCPTP
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length Human TCPTP purified from E.coli.

  • Positive control

    • Bosc23 cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.03% Sodium azide
    Constituents: 0.87% Sodium chloride, HEPES, 0.01% BSA, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Clonality

    Monoclonal
  • Clone number

    T25-AF3B7
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Phosphatases

Images

  • Western blot - Anti-TCPTP antibody [T25-AF3B7] (ab85330)
    Western blot - Anti-TCPTP antibody [T25-AF3B7] (ab85330)
    All lanes : Anti-TCPTP antibody [T25-AF3B7] (ab85330) at 1/2000 dilution

    Lane 1 : Bosc23 Cell Lysate
    Lane 2 : Bosc23 Cell Lysate Transfected with Myc/TCPTP
    Lane 3 : Bosc23 Cell Lysate Transfected with Myc/Cdc25A
    Lane 4 : Bosc23 Cell Lysate Transfected with GFP/Ab40

    Predicted band size: 49 kDa



    Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

  • Western blot - Anti-TCPTP antibody [T25-AF3B7] (ab85330)
    Western blot - Anti-TCPTP antibody [T25-AF3B7] (ab85330)
    All lanes : Anti-TCPTP antibody [T25-AF3B7] (ab85330) at 1/2000 dilution

    Lane 1 : Bosc23 cell lysate
    Lane 2 : Bosc23 cell lysate transfected with Myc/His/Tau (1-441 aa)
    Lane 3 : Bosc23 cell lysate transfected with Myc/His/TCPTP (1-387aa)
    Lane 4 : Bosc23 cell lysate transfected with GFP-Ub+1

    Predicted band size: 49 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-TCPTP antibody [T25-AF3B7] (ab85330)

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    Anti-TCPTP antibody (ab1996)

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    Anti-TCPTP antibody (ab227916)

    Applications: IHC-P, IP, WB

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    Anti-TCPTP antibody (ab180764)

    Applications: ICC/IF, IHC-P, WB

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    Anti-TCPTP antibody (ab264379)

    Applications: IP

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    Anti-TCPTP antibody [EPR6712] (ab129070)

    Applications: Flow Cyt, IP, WB

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