Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-TCPTP antibody [EPR6712] (ab129070)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6712] to TCPTP
Suitable for: Flow Cyt, WB, IP
Reacts with: Mouse, Human

Product name

Anti-TCPTP antibody [EPR6712]
See all TCPTP primary antibodies
Description

Rabbit monoclonal [EPR6712] to TCPTP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human TCPTP aa 1-100. The exact sequence is proprietary.
Positive control
- HL60, NIH 3T3, MOLT4 and HepG2 whole cell lysate (ab7900); Mouse placenta and thymus lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6712
Isotype

IgG
Research areas

Western blot - Anti-TCPTP antibody [EPR6712] (ab129070)

All lanes : Anti-TCPTP antibody [EPR6712] (ab129070) at 1/1000 dilution

Lane 1 : HL60 cell lysate
Lane 2 : NIH 3T3 cell lysate
Lane 3 : Mouse placenta lysate
Lane 4 : Mouse thymus lysate
Lane 5 : MOLT4 cell lysate
Lane 6 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 48 kDa
Observed band size: 45-48 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-TCPTP antibody [EPR6712] (ab129070)

Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) cell line fixed with 4% paraformaldehyde and permeabilized with 90% methanol labeling TCPTP with ab129070 at 1:200 dilution (red). This is compared with a Rabbit monoclonal IgG – Isotype control (ab172730) (black) and a unalbelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-rabbit IgG (Alexa Fluor^®488) (ab150077) at 1:2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-TCPTP antibody [EPR6712] (ab129070)

Lane 1 (input): MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate, 10μg
Lane 2 (+): MOLT-4 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129070 in MOLT-4 whole cell lysate.

Ab129070 Immunoprecipitating TCPTP in MOLT-4 whole cell lysate. Capture antibody was used at a 1:20 dilution (0.6μg in 0.35mg lysates). For western blotting, primary antibody used was ab129070 at 1:1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking and diluting buffer: 5% NFDM/TBST

Exposure time: 1second.
Anti-TCPTP antibody [EPR6712] (ab129070)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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