Anti-LDH-C antibody [EP1746Y] (ab52747)
Key features and details
- Rabbit monoclonal [EP1746Y] to LDH-C
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LDH-C antibody [EP1746Y]
See all LDH-C primary antibodies -
Description
Rabbit monoclonal [EP1746Y] to LDH-C -
Host species
Rabbit -
Specificity
This antibody reacts with Lactate Dehydrogenase C / LDH-C. The immunogen sequence is 100% identical to Lactate Dehydrogenase C / LDH-C, but there is the possibility of cross-reactivity for lactate dehydrogenase A and lactate dehydrogenase B at 69% and 82%, respectively. -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human LDH-C aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P07864 -
Positive control
- WB: Human testis lysate. IHC-P: Human testis carcinoma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Lactate Dehydrogenase C.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1746Y -
Isotype
IgG -
Research areas
Images
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Anti-LDH-C antibody [EP1746Y] (ab52747) at 1/20000 dilution + Human testis lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
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Immunohistochemical staining of paraffin-embedded human testis carcinoma using ab52747 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.