Human Oncostatin M/OSM Matched Antibody Pair Kit (ab218803)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 7.8 pg/ml - 500 pg/ml
Overview
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Product name
Human Oncostatin M/OSM Matched Antibody Pair Kit
See all Oncostatin M/OSM kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
2.3 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Oncostatin M Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Oncostatin M.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab215543. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Oncostatin M Capture Antibody 2 x 50µg 1 x 50µg Human Oncostatin M Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Oncostatin M Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). -
Sequence similarities
Belongs to the LIF/OSM family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- MGC20461
- ONCM_HUMAN
- Oncostatin M
see all -
Database links
- Entrez Gene: 5008 Human
- Omim: 165095 Human
- SwissProt: P13725 Human
- Unigene: 248156 Human
Images
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Human Oncostatin M standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Oncostatin M/OSM Matched Antibody Pair Kit (ab218803)To learn more about the advantages of recombinant antibodies see here.
