Anti-FSTL1/FRP antibody (ab111969)
Key features and details
- Rabbit polyclonal to FSTL1/FRP
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FSTL1/FRP antibody
See all FSTL1/FRP primary antibodies -
Description
Rabbit polyclonal to FSTL1/FRP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Dog, Chimpanzee, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Human Plasma Total Protein lysate as well as the following whole cell lysates: A431; MD MBA 231; HeLa; NIH3T3.
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General notes
This product was previously labelled as FSTL1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab111969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 48, 50 kDa (predicted molecular weight: 35 kDa). Target
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Function
May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin. -
Tissue specificity
Overexpressed in synovial tissues from rheumatoid arthritis. -
Sequence similarities
Contains 2 EF-hand domains.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 534482 Cow
- Entrez Gene: 11167 Human
- Entrez Gene: 14314 Mouse
- Entrez Gene: 100172767 Orangutan
- Entrez Gene: 79210 Rat
- Omim: 605547 Human
- SwissProt: Q58D84 Cow
- SwissProt: Q12841 Human
see all -
Alternative names
- Anti FSTL1 antibody
- FLJ50214 antibody
- FLJ52277 antibody
see all
Images
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Western blot - Anti-FSTL1/FRP antibody (ab111969)All lanes : Anti-FSTL1/FRP antibody (ab111969) at 1 µg/ml
Lane 1 : Human Plasma Total Protein Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 11 kDa (possible non-specific binding), 48 kDa (possible cleavage fragment), 95 kDa (possible non-specific binding)
Exposure time: 2 minutesFSTL1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
The band observed at 48 kDa could potentially be a cleaved form of FSTL1 due to the presence of a 20amino acid signal peptide.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111969 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab111969 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-FSTL1/FRP antibody (ab111969)All lanes : Anti-FSTL1/FRP antibody (ab111969) at 1 µg/ml
Lane 1 : Human Plasma Total Protein Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 11 kDa (possible non-specific binding), 48 kDa (possible cleavage fragment), 95 kDa (possible non-specific binding)
Exposure time: 2 minutesFSTL1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
The band observed at 48 kDa could potentially be a cleaved form of FSTL1 due to the presence of a 20amino acid signal peptide.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111969 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
