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Human Ghrelin ELISA Kit (Ghrelin-28) (ab263887)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.048 pg/ml
  • Range: 1.95 pg/ml - 125 pg/ml
  • Sample type: Cell culture media, Cit plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Ghrelin ELISA Kit (Ghrelin-28)
    See all Ghrelin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 5.6%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6.1%

  • Sample type

    Serum, Cell culture media, Hep Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.048 pg/ml

  • Range

    1.95 pg/ml - 125 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 101 97% - 105%
    Cell culture media 97 96% - 99%
    Hep Plasma 99 96% - 101%
    Cit plasma 78 73% - 85%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Cow

  • Product overview

    Human Ghrelin ELISA Kit (Ghrelin-28) (ab263887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Ghrelin (Ghrelin-28) protein in cell culture media, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Ghrelin (Ghrelin-28) with 1.048 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Ghrelin is a peptide hormone, it is a ligand of growth hormone secretagogue receptor type 1 (GHSR). It is involved in appetite stimulation and gastric acid secretion and induces adiposity. It is involved in growth regulation and induces the release of growth hormone from the pituitary. Ghrelin exists in two forms, Ghrelin-28 and one amino acid shorter Ghrelin-27. Both forms as well as Obestatin are generated by cleavage from common precursor protein, Appetite-Regulating Hormone coded by GHRL gene. Ghrelin-28 and Obestatin have non-overlapping amino acid sequences.  O-octanoylation or O-decanoylation of Ghrelins is essential for Ghrelin activity.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Ghrelin (Ghrelin-28) Capture Antibody 1 x 600µl
    10X Human Ghrelin (Ghrelin-28) Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human Ghrelin (Ghrelin-28) Lyophilized Peptide Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.

  • Tissue specificity

    Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.

  • Sequence similarities

    Belongs to the motilin family.

  • Post-translational
    modifications

    O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Appetite regulating hormone
    • Ghrelin
    • Ghrelin 27
    • Ghrelin 28
    • Ghrelin and obestatin prepropeptide
    • Ghrelin/obestatin prepropeptide
    • ghrl
    • GHRL_HUMAN
    • Growth hormone releasing peptide
    • Growth hormone secretagogue
    • Growth hormone-releasing peptide
    • M46 protein
    • Motilin related peptide
    • Motilin-related peptide
    • MTLRP
    • Obestatin
    • Protein M46
    see all

  • Database links

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of human Ghrelin standard curve in Sample Diluent NS.
      Example of human Ghrelin standard curve in Sample Diluent NS.

      The Ghrelin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native Ghrelin in human serum and plasma samples.
      Interpolated concentrations of native Ghrelin in human serum and plasma samples.

      The concentrations of Ghrelin were measured in duplicates, interpolated from the Ghrelin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ghrelin concentration was determined to be 25.69 pg/mL in neat serum and 31.66 pg/mL in neat plasma (citrate).

    • Serum from ten individual healthy human female donors was measured in duplicate.
      Serum from ten individual healthy human female donors was measured in duplicate.

      Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ghrelin concentration of detectable samples was determined to be 13.42 pg/mL with a range of undetectable – 36.06 pg/mL in all samples.

    • Reactivity comparison of Ghrelin-28 (acylated peptide) and [Des-octanoyl]-Ghrelin-28.
      Reactivity comparison of Ghrelin-28 (acylated peptide) and [Des-octanoyl]-Ghrelin-28.

      [Des-octanoyl]-Ghrelin-28 is the non-acylated major circulating isoform of Ghrelin.

    • Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (ab263887)
      Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (ab263887)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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