Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HSPA4 antibody [EPR14165] (ab185219)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14165] to HSPA4
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HSPA4 antibody [EPR14165]
    See all HSPA4 primary antibodies

  • Description

    Rabbit monoclonal [EPR14165] to HSPA4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562 cells and cell lysates; HepG2 cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HSPA4 antibody [EPR14165] (ab185219)
    Western blot - Anti-HSPA4 antibody [EPR14165] (ab185219)
    All lanes : Anti-HSPA4 antibody [EPR14165] (ab185219) at 1/5000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 94 kDa

  • Flow Cytometry - Anti-HSPA4 antibody [EPR14165] (ab185219)
    Flow Cytometry - Anti-HSPA4 antibody [EPR14165] (ab185219)

    Flow cytometric analysis of 2% paraformaldehyde fixed K562 cells labeling HSPA4 with ab185219 at 1/300 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] (ab185219)
    Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] (ab185219)

    Western blot analysis of immunoprecipitation pellet from K562 cell lysate labeling HSPA4 using ab185219 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at a 1/1000 dilution.

  • Anti-HSPA4 antibody [EPR14165] (ab185219)
    Anti-HSPA4 antibody [EPR14165] (ab185219)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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