Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 10, 2021

Anti-HSPA4 antibody [EPR14165] (ab185219)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14165] to HSPA4
Suitable for: Flow Cyt, IP, WB
Reacts with: Human

Product name

Anti-HSPA4 antibody [EPR14165]
See all HSPA4 primary antibodies
Description

Rabbit monoclonal [EPR14165] to HSPA4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- K562 cells and cell lysates; HepG2 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14165
Isotype

IgG
Research areas

Western blot - Anti-HSPA4 antibody [EPR14165] (ab185219)

All lanes : Anti-HSPA4 antibody [EPR14165] (ab185219) at 1/5000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 94 kDa
Flow Cytometry - Anti-HSPA4 antibody [EPR14165] (ab185219)

Flow cytometric analysis of 2% paraformaldehyde fixed K562 cells labeling HSPA4 with ab185219 at 1/300 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] (ab185219)

Western blot analysis of immunoprecipitation pellet from K562 cell lysate labeling HSPA4 using ab185219 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at a 1/1000 dilution.
Anti-HSPA4 antibody [EPR14165] (ab185219)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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