Human Flt-3 ligand ELISA Kit (ab254507)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2 pg/ml
- Range: 4.7 pg/ml - 300 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Flt-3 ligand ELISA Kit
See all Flt3 ligand/Flt3L kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.1% Inter-assay Sample n Mean SD CV% Serum 3 4.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
4.7 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 94% - 97% Serum 99 93% - 103% Hep Plasma 98 93% - 101% EDTA Plasma 94 87% - 97% Cit plasma 97 93% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Flt-3 ligand ELISA Kit (ab254507) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Flt-3 ligand protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Flt-3 ligand with 2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Flt-3 Ligand binds to and activates Flt-3. Flt-3 Ligand works synergistically with other CSFs and interleukins to stimulate growth and differentiation of early hematopoietic cells. The predominant form of Flt-3 Ligand is the biologically active transmembrane form which is proteolytically cleaved to generate a soluble form that is also biologically active. Alternative spliced secreted soluble variant of Flt-3 Ligand has also been identified.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Flt-3 ligand Capture Antibody 1 x 600µl 10X Human Flt-3 ligand Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Flt-3 ligand Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- FL
- Flt 3 ligand
- Flt 3L
see all -
Database links
- Entrez Gene: 2323 Human
- Omim: 600007 Human
- SwissProt: P49771 Human
- Unigene: 428 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Fit-3 Ligand standard curve comparisonStandard Curve comparison between human Fit-3 Ligand SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human Flt-3 Ligand standard curve in Sample Diluent NS.The Flt-3 Ligand standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Flt-3 Ligand in human serum and plasma samples.The concentrations of Flt-3 Ligand were measured in duplicates, interpolated from the Flt-3 Ligand standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Flt-3 Ligand concentration was determined to be 65.35 pg/mL in neat serum, 57.01 pg/mL in neat plasma (citrate), 54.11 pg/mL in neat plasma (heparin), and 64.57 pg/mL in neat plasma (EDTA).
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Interpolated concentrations of native Flt-3 Ligand in human PBMC cell culture supernatant samples. Human PBMC were cultured in the presence (stimulated) or absence (unstimulated) of 1.5% PHA-M.Human peripheral blood mononuclear cells (PBMC) were cultured in the presence (stimulated) or absence (unstimulated) of 1.5% PHA-M for 36 hours. The concentrations of Flt-3 Ligand were measured in duplicates, interpolated from the Flt-3 Ligand standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC supernatant 100% and unstimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Flt-3 Ligand concentration was determined to be 22.72 pg/mL in neat PHA-M stimulated PBMC cell culture supernatant, 3.742 pg/mL in neat unstimulated PBMC cell culture supernatant, and undetectable in naïve cell culture media (data not shown).
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Flt-3 Ligand concentration was determined to be 63.87 pg/mL with a range of 26.63 – 105.1 pg/mL.
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Sandwich ELISA - Human Flt-3 ligand ELISA Kit (ab254507)To learn more about the advantages of recombinant antibodies see here.
