Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp70 antibody [EPR16893] (ab194360)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16893] to Hsp70
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Hsp70 antibody [EPR16893]
    See all Hsp70 primary antibodies

  • Description

    Rabbit monoclonal [EPR16893] to Hsp70

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HepG2, Jurkat, C6, PC12, human testis, mouse testis and rat testis lysates; Human endometrium tissue; A431 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16893

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/2000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR16893] (ab194360)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Hsp70 with ab194360 at 1/1000 (1.67μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [EPR16893] (ab194360)

    Immunofluorescent analysis of A431 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Hsp70 with ab194360 at 1/150 dilution (11 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).

  • Flow Cytometry - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Flow Cytometry - Anti-Hsp70 antibody [EPR16893] (ab194360)

    Flow cytometry analysis of HeLa cells labelling Hsp70 (red) with purified ab194360 at dilution of 1/120. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/2000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Anti-Hsp70 antibody [EPR16893] (ab194360)
    Anti-Hsp70 antibody [EPR16893] (ab194360)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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