Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Hsp70 antibody [EPR16893] (ab194360)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16893] to Hsp70
Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Hsp70 antibody [EPR16893]
See all Hsp70 primary antibodies
Description

Rabbit monoclonal [EPR16893] to Hsp70
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, HepG2, Jurkat, C6, PC12, human testis, mouse testis and rat testis lysates; Human endometrium tissue; A431 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16893
Isotype

IgG
Research areas

Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)

All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/2000 dilution

Lane 1 : C6 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR16893] (ab194360)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Hsp70 with ab194360 at 1/1000 (1.67μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [EPR16893] (ab194360)

Immunofluorescent analysis of A431 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Hsp70 with ab194360 at 1/150 dilution (11 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).
Flow Cytometry - Anti-Hsp70 antibody [EPR16893] (ab194360)

Flow cytometry analysis of HeLa cells labelling Hsp70 (red) with purified ab194360 at dilution of 1/120. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)

All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/2000 dilution

Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 70 kDa
Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)

All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/20000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 70 kDa
Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)

All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/20000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Human testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 70 kDa
Anti-Hsp70 antibody [EPR16893] (ab194360)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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