Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Hsp70 antibody [C92F3A-5] (ab47455)

Key features and details

Mouse monoclonal [C92F3A-5] to Hsp70
Suitable for: WB, Flow Cyt, IHC-P
Reacts with: Mouse, Rat, Human
Isotype: IgG1

Product name

Anti-Hsp70 antibody [C92F3A-5]
See all Hsp70 primary antibodies
Description

Mouse monoclonal [C92F3A-5] to Hsp70
Host species

Mouse
Specificity

Detects a 70kDa protein corresponding to the molecular mass of Hsp70 of SDS PAGE immunoblots. There is no cross-reactivity to Hsc70 (Hsp73).
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human Hsp70 aa 436-503.
Sequence:
YSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDI DANGILNVTATDKSTGKANKITI

Database link: P08107
Run BLAST with Run BLAST with
Epitope

The mapped epitope is in the region of amino acid residues 436-503.
Positive control
- WB: HeLa, A431, A549, HCT116, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3, T98G cell lysates; Rat Brain tumour lysate; Rat bone lysate. IHC-P: Mouse colon cancer tissue; human colon cancer tissue. Flow cyt: Heat Shocked CD3+ CD8+ T cells

Application	Species
IHC-P	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

C92F3A-5
Isotype

IgG1
Research areas

Western blot - Anti-Hsp70 antibody [C92F3A-5] (ab47455)

All lanes : Anti-Hsp70 antibody [C92F3A-5] (ab47455) at 1 µg/ml

Lane 1 : Cell lysate prepared from human A431 cell line
Lane 2 : Cell lysate prepared from human A549 cell line
Lane 3 : Cell lysate prepared from human HCT116 cells line
Lane 4 : Cell lysate prepared from human Hela cell line
Lane 5 : Cell lysate prepared from human HEK293 cell line
Lane 6 : Cell lysate prepared from human HepG2 cell line
Lane 7 : Cell lysate prepared from human HL-60 cell line
Lane 8 : Cell lysate prepared from human HUVEC cell line
Lane 9 : Cell lysate prepared from human Jurkat cell line
Lane 10 : Cell lysate prepared from human MCF7 cell line
Lane 11 : Cell lysate prepared from human PC3 cell line
Lane 12 : Cell lysate prepared from human T98G cell line
Lane 13 : Rat brain tissue lysates

Predicted band size: 70 kDa

All are cancer cell lines.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [C92F3A-5] (ab47455)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Hsp70 using ab47455 at 1/1000 dilution in immunohistochemical analysis. Counter stained with hematoxylin.
Western blot - Anti-Hsp70 antibody [C92F3A-5] (ab47455) Image courtesy of an anonymous AbReview

All lanes : Anti-Hsp70 antibody [C92F3A-5] (ab47455) at 1/500 dilution

All lanes : Rat bone (tibia) whole cell lysate

Lysates/proteins at 50 µg per lane.

Predicted band size: 70 kDa

See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [C92F3A-5] (ab47455)

ab47455 staining Hsp70 in mouse colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). The primary antibody was diluted at 1/100,000. A Fluorophore conjugated goat anti mouse was used as secondary. An antibody amplifier™ system was used for staining.
Flow Cytometry - Anti-Hsp70 antibody [C92F3A-5] (ab47455)

FACS analysis using ab47455 staining heat shock treated CD3 + CD8 + T cells.

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