Antibodies, Proteins, Kits and Reagents for Life Science

301 536 ₸ Product size

100 µl 301 536 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Rabbit polyclonal to Hsp70
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Mouse, Human
Isotype: n/a

Product name

Anti-Hsp70 antibody
See all Hsp70 primary antibodies
Description

Rabbit polyclonal to Hsp70
Host species

Rabbit
Specificity

Detects a 70kDa protein corresponding to the molecular mass of inducible hsp70. May cross-react with Hsc70 at lower dilutions.

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Full length protein corresponding to Human Hsp70 aa 1-641.
Sequence:
MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERL IGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDK PKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYF NDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDL GGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHK KDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRA RFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLL QDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLS LGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMT KDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANK ITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMK SAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELE QVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD

Database link: P08107
Run BLAST with Run BLAST with
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Research areas

Western blot - Anti-Hsp70 antibody (ab79852)

All lanes : Anti-Hsp70 antibody (ab79852) at 1/25000 dilution

Lane 1 : Cell lysates prepared from mouse Pam212 cells
Lane 2 : Molecular weight marker
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab79852)

Immunohistochemistry of human colon carcinoma staining Hsp70 using ab79852 at 1:50000. A Biotin conjugated Goat Anti-Rabbit antibody at 1:2000 was used as a secondary antibody and Methyl Green at 200uL was used to counter stain.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (ab79852)

Immunocytochemistry/Immunofluorescence analysis of heat shocked HeLa cells staining Hsp70 using ab79852 at a 1:100 dilution. The secondary antibody was a FITC conjugated Goat Anti-Rabbit (green) at a 1:200 dilution. Counterstain: DAPI (blue) nuclear stain at 1:40000. A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab79852)

ab79852 staining Hsp70 in mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western blot - Anti-Hsp70 antibody (ab79852)

All lanes : Anti-Hsp70 antibody (ab79852) at 1/25000 dilution

Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human A431 cells
Lane 3 : Cell lysates prepared from human A549 cells
Lane 4 : Cell lysates prepared from human HCT116 cells
Lane 5 : Cell lysates prepared from Hela cells
Lane 6 : Cell lysates prepared from HEK293 cells
Lane 7 : Cell lysates prepared from HepG2 cells
Lane 8 : Cell lysates prepared from HL-60 cells
Lane 9 : Cell lysates prepared from HUVEC cells
Lane 10 : Cell lysates prepared from Jurkat cells
Lane 11 : Cell lysates prepared from MCF7 cells
Lane 12 : Cell lysates prepared from PC3 cells
Lane 13 : Cell lysates prepared from T98G cells
Lane 14 : Tissue lysates prepared from Rat brain

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