Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp70 antibody (ab79852)

Key features and details

  • Rabbit polyclonal to Hsp70
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: n/a

Overview

  • Product name

    Anti-Hsp70 antibody
    See all Hsp70 primary antibodies

  • Description

    Rabbit polyclonal to Hsp70

  • Host species

    Rabbit

  • Specificity

    Detects a 70kDa protein corresponding to the molecular mass of inducible hsp70. May cross-react with Hsc70 at lower dilutions.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Full length protein corresponding to Human Hsp70 aa 1-641.
    Sequence:

    MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERL IGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDK PKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYF NDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDL GGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHK KDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRA RFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLL QDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLS LGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMT KDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANK ITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMK SAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELE QVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD


    Database link: P08107
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Hsp70 antibody (ab79852)
    Western blot - Anti-Hsp70 antibody (ab79852)
    All lanes : Anti-Hsp70 antibody (ab79852) at 1/25000 dilution

    Lane 1 : Cell lysates prepared from mouse Pam212 cells
    Lane 2 : Molecular weight marker
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab79852)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab79852)

    Immunohistochemistry of human colon carcinoma staining Hsp70 using ab79852 at 1:50000. A Biotin conjugated Goat Anti-Rabbit antibody at 1:2000 was used as a secondary antibody and Methyl Green at 200uL was used to counter stain.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (ab79852)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (ab79852)

    Immunocytochemistry/Immunofluorescence analysis of heat shocked HeLa cells staining Hsp70 using ab79852 at a 1:100 dilution. The secondary antibody was a FITC conjugated Goat Anti-Rabbit (green) at a 1:200 dilution. Counterstain: DAPI (blue) nuclear stain at 1:40000. A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab79852)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab79852)

    ab79852 staining Hsp70 in mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

  • Western blot - Anti-Hsp70 antibody (ab79852)
    Western blot - Anti-Hsp70 antibody (ab79852)
    All lanes : Anti-Hsp70 antibody (ab79852) at 1/25000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from human A431 cells
    Lane 3 : Cell lysates prepared from human A549 cells
    Lane 4 : Cell lysates prepared from human HCT116 cells
    Lane 5 : Cell lysates prepared from Hela cells
    Lane 6 : Cell lysates prepared from HEK293 cells
    Lane 7 : Cell lysates prepared from HepG2 cells
    Lane 8 : Cell lysates prepared from HL-60 cells
    Lane 9 : Cell lysates prepared from HUVEC cells
    Lane 10 : Cell lysates prepared from Jurkat cells
    Lane 11 : Cell lysates prepared from MCF7 cells
    Lane 12 : Cell lysates prepared from PC3 cells
    Lane 13 : Cell lysates prepared from T98G cells
    Lane 14 : Tissue lysates prepared from Rat brain

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