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Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14457] to Hsp20 - N-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Hsp20 antibody [EPR14457] - N-terminal
    See all Hsp20 primary antibodies

  • Description

    Rabbit monoclonal [EPR14457] to Hsp20 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle and rat heart lysates; Human transitional cell carcinoma of bladder tissue; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864) at 1/1000 dilution + Rat heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/500 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling Hsp20 with ab188864 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Hsp20 with ab188864 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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