Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp20 antibody [EPR14458] (ab184161)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14458] to Hsp20
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Hsp20 antibody [EPR14458]
    See all Hsp20 primary antibodies

  • Description

    Rabbit monoclonal [EPR14458] to Hsp20

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle lysate; Human and mouse skeletal muscle tissues; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Hsp20 antibody [EPR14458] (ab184161)
    Western blot - Anti-Hsp20 antibody [EPR14458] (ab184161)
    Anti-Hsp20 antibody [EPR14458] (ab184161) at 1/50000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14458] (ab184161)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14458] (ab184161)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Hsp20 with ab184161 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14458] (ab184161)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14458] (ab184161)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Hsp20 with ab184161 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Hsp20 antibody [EPR14458] (ab184161)
    Flow Cytometry - Anti-Hsp20 antibody [EPR14458] (ab184161)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Hsp20 with ab184161 at 1/100 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green) followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Anti-Hsp20 antibody [EPR14458] (ab184161)
    Anti-Hsp20 antibody [EPR14458] (ab184161)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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