Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CLPTM1 antibody [EPR8800] (ab133756)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8800] to CLPTM1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CLPTM1 antibody [EPR8800]
    See all CLPTM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8800] to CLPTM1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CLPTM1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O96005

  • Positive control

    • WB: A549, HAP1, HeLa, 293T, U87 MG, HepG2 and Jurkat cell lysate, IHC: Papillary adenocarcinoma of Human thyroid gland tissue. ICC/IF: HeLa cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 3.45 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8800

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CLPTM1 antibody [EPR8800] (ab133756)
    Western blot - Anti-CLPTM1 antibody [EPR8800] (ab133756)
    All lanes : Anti-CLPTM1 antibody [EPR8800] (ab133756) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CLPTM1 knockout HAP1 whole cell lysate
    Lane 3 : A549 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 76 kDa
    Observed band size: 76 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab133756 observed at 76 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133756 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in CLPTM1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CLPTM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3pc Milk. Ab133756 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CLPTM1 antibody [EPR8800] (ab133756)
    Western blot - Anti-CLPTM1 antibody [EPR8800] (ab133756)
    All lanes : Anti-CLPTM1 antibody [EPR8800] (ab133756) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : U87 MG cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 76 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPTM1 antibody [EPR8800] (ab133756)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPTM1 antibody [EPR8800] (ab133756)

    Immunohistochemical analysis of paraffin embedded papillary adenocarcinoma of Human thyroid gland tissue labelling CLPTM1 with ab133756 at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CLPTM1 antibody [EPR8800] (ab133756)
    Immunocytochemistry/ Immunofluorescence - Anti-CLPTM1 antibody [EPR8800] (ab133756)
    Immunofluorescent staining of HeLa cells labelling CLPTM1 with ab133756 at 1/250.
  • OI-RD Scanning - Anti-CLPTM1 antibody [EPR8800] (ab133756)
    OI-RD Scanning - Anti-CLPTM1 antibody [EPR8800] (ab133756)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CLPTM1 antibody [EPR8800] (ab133756)
    Anti-CLPTM1 antibody [EPR8800] (ab133756)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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