Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7266(2)] to SCDGFB/PDGF-D
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SCDGFB/PDGF-D antibody [EPR7266(2)]
    See all SCDGFB/PDGF-D primary antibodies

  • Description

    Rabbit monoclonal [EPR7266(2)] to SCDGFB/PDGF-D

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney, fetal heart and small intestine lysate. PC-3 cell lysate. Human squamous cell lung carcinoma tissue. Mouse heart tissue.

  • General notes

    Previously labeled as SCDGFB. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Western blot - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    All lanes : Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human small intestine lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)

    Predicted band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)

    Immunohistochemical analysis of paraffin-embedded Mouse heart tissue labeling SCDGFB/PDGF-D using ab181845 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG prediluted was used as secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)

    Immunohistochemical analysis of paraffin-embedded Human squamous cell lung carcinoma tissue labeling SCDGFB/PDGF-D using ab181845 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG prediluted was used as secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Western blot - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845) at 1/1000 dilution + PC3 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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