Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420)
![Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420)](https://www.abcam.com/ps/products/191/ab191420/Images/ab191420-261769-anti-samhd1-antibody-epr13034-n-terminal-western-blot.jpg "Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13034] to SAMHD1 - N-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SAMHD1 antibody [EPR13034] - N-terminal
See all SAMHD1 primary antibodies -
Description
Rabbit monoclonal [EPR13034] to SAMHD1 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- THP1, Daudi, HeLa and HepG2 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13034 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SAMHD1 knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab191420 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab191420 was shown to recognize SAMHD1 when SAMHD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and SAMHD1 knockout samples were subjected to SDS-PAGE. ab191420 and ab8245 (loading control to GAPDH) dilted to 1/500 and 1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420)](https://www.abcam.com/ps/products/191/ab191420/Images/ab191420-228792-anti-samhd1-antibody-epr13034-n-terminal-western-blot.jpg)
Western blot - Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420)Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420) at 1/10000 dilution + THP1 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 72 kDa
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![Western blot - Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420)](https://www.abcam.com/ps/products/191/ab191420/Images/ab191420-228793-anti-samhd1-antibody-epr13034-n-terminal-western-blot.jpg)
Western blot - Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420)All lanes : Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 72 kDa
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![Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420)](https://www.abcam.com/ps/products/191/ab191420/Images/ab191420-4-benefits-of-recombinant-antibodies.png)
Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420)
