Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13040(B)] to SAMHD1 - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal
    See all SAMHD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13040(B)] to SAMHD1 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SAMHD1 aa 600 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y3Z3

  • Positive control

    • THP1, K562, HeLa and MCF7 cell lysates. Human pancreas and tonsil tissue. MCF7 and THP1 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13040(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)
    Western blot - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SAMHD knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab177462 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab177462 was shown to recognize SAMHD1 when SAMHD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and SAMHD1 knockout samples were subjected to SDS-PAGE. ab177462 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SAMHD1 with ab177462 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)
    Immunocytochemistry/ Immunofluorescence - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)

    Immunofluorescence analysis of MCF7 cells labeling SAMHD1 with ab177462 at 1/100 dilution.

  • Flow Cytometry - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)
    Flow Cytometry - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)

    Flow cytometric analysis of permeabilized THP1 cells labeling SAMHD1 (red) with ab177462 at 1/10 dilution or  rabbit IgG (negative) (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SAMHD1 with ab177462 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)
    Western blot - Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)
    All lanes : Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462) at 1/10000 dilution

    Lane 1 : THP1 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 72 kDa

  • Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)
    Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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