Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17677] to Hsp70 - BSA and Azide free
  • Suitable for: IP, IHC-P, Flow Cyt, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Hsp70 antibody [EPR17677] - BSA and Azide free
    See all Hsp70 primary antibodies

  • Description

    Rabbit monoclonal [EPR17677] to Hsp70 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, Flow Cyt, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250639 is the carrier-free version of ab182844. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250639 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    Immunocytochemistry - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)

    This data was developed using ab182844, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling Hsp70 with ab182844 at 1/100 dilution, followed by Alexa Fluor®488 Goat Anti-Rabbit IgG H&L (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing both cytoplasmic and nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab182844 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Western blot - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    Western blot - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    All lanes : Anti-Hsp70 antibody [EPR17677] (ab182844) at 1/50000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate untreated at 37°C
    Lane 2 : MCF7 whole cell lysate heat shock (43°C) treated for 1 hour
    Lane 3 : MCF7 whole cell lysate heat shock (43°C) treated for 2 hours
    Lane 4 : MCF7 whole cell lysate heat shock (43°C) treated for 3 hours
    Lane 5 : MCF7 whole cell lysate heat shock (43°C) treated for 4 hours
    Lane 6 : MCF7 whole cell lysate heat shock (43°C) treated for 5 hours
    Lane 7 : MCF7 whole cell lysate heat shock (43°C) treated for 6 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa


    Exposure time: 30 seconds


    This data was developed using ab182844, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    Western blot - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    All lanes : Anti-Hsp70 antibody [EPR17677] (ab182844) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : A431 (Human epidermoid carcinoma) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    This data was developed using ab182844, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    Western blot - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    All lanes : Anti-Hsp70 antibody [EPR17677] (ab182844) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    This data was developed using ab182844, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)

    This data was developed using ab182844, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Hsp70 with ab182844 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on epithelial cells of Human mammary glands is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    This data was developed using ab182844, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Hsp70 with ab182844 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on epithelial cells of Human prostate is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    This data was developed using ab182844, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung squamous cell carcinoma tissue labeling Hsp70 with ab182844 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on cancer cells of Human lung squamous cell carcinoma is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    This data was developed using ab182844, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling Hsp70 with ab182844 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on cancer cells of Human transitional cell carcinoma of bladder is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    Flow Cytometry - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    This data was developed using ab182844, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Hsp70 with ab182844 at 1/30 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    Immunoprecipitation - Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    This data was developed using ab182844, the same antibody clone in a different buffer formulation.Hsp70 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab182844 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab182844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HeLa whole cell extract (Input) 10 µg.Lane 2: ab182844 IP in HeLa whole cell extract.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182844 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  • Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)
    Anti-Hsp70 antibody [EPR17677] - BSA and Azide free (ab250639)

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