Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17684(2)] to Histone H3 (di methyl K56) - BSA and Azide free
  • Suitable for: PepArr, ICC, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [EPR17684(2)] to Histone H3 (di methyl K56) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: PepArr, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249955 is the carrier-free version of ab177294. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249955 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17684(2)

  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
    Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
    Lane 1 : Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] (ab177294) at 1/5000 dilution
    Lanes 2-4 : Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] (ab177294) at 1/1000 dilution

    Lane 1 : Recombinant mutant H3 chemically modified to mimic H3K56Me2
    Lane 2 : Unmodified recombinant mutant H3
    Lane 3 : Recombinant mutant H3 chemically modified to mimic H3K56Me1
    Lane 4 : Recombinant mutant H3 chemically modified to mimic H3K56Me3

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 3 minutes


    This data was developed using ab177294, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% BSA/TBST.

  • Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
    Western blot - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
    All lanes : Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] (ab177294) at 1/200 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 3 minutes


    This data was developed using ab177294, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Peptide Array - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
    Peptide Array - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
    This data was developed using ab177294, the same antibody clone in a different buffer formulation.ab177294 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
  • Immunocytochemistry - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
    Immunocytochemistry - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
    This data was developed using ab177294, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (di methyl K56) with ab177294 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution (red). The negative controls are as follows:
    -ve control 1: ab177294 at 1/150 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Immunocytochemistry - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
    Immunocytochemistry - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
    This data was developed using ab177294, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Histone H3 (di methyl K56) with ab177294 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution (red). The negative controls are as follows:
    -ve control 1: ab177294 at 1/150 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)
    Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] - BSA and Azide free (ab249955)

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