Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22933-9] to non-muscle Myosin IIA
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-non-muscle Myosin IIA antibody [EPR22933-9]
See all non-muscle Myosin IIA primary antibodies
Description

Rabbit monoclonal [EPR22933-9] to non-muscle Myosin IIA
Host species

Rabbit
Specificity

IHC is not recommended for human.

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human liver and lung tissue lysates; mouse lung tissue lysate; rat spleen and lung tissue lysates; Neuro-2a, HEK-293T and HeLa whole cell lysates; IP: NIH/3T3 whole cell lysate; IHC-P: Mouse liver tissue; rat liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22933-9
Isotype

IgG
Research areas

Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

All lanes : Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131) at 1/1000 dilution

Lane 1 : Human liver tissue lysate 20ug
Lane 2 : Human lung lysate at 20 µg
Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg
Lane 4 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 5 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 6 : Mouse lung tissue lysate at 20 µg
Lane 7 : Rat spleen tissue lysate at 20 µg
Lane 8 : Rat lung tissue lysate at 20 µg

Secondary
Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 4-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 226 kDa
Observed band size: 226 kDa

Exposure time: 26 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

non-muscle Myosin IIA was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug with ab238131 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238131. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug

Lane 2: ab238131 IP in NIH/3T3 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238131 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling non-muscle Myosin IIA with ab238131 at 1/500 dilution (0.854 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat liver (PMID: 11943476, 22558294, 17668046). The section was incubated with ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling non-muscle Myosin IIA with ab238131 at 1/500 dilution (0.854 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse liver (PMID: 11943476, 22558294, 17668046). The section was incubated with ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

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