Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17043(B)] to Ribophorin I - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free
    See all Ribophorin I primary antibodies

  • Description

    Rabbit monoclonal [EPR17043(B)] to Ribophorin I - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human placenta tissue.

  • General notes

    Ab232599 is the carrier-free version of ab198508. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232599 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17043(B)

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)
    Flow Cytometry - Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)

    Flow cytometry analysis of HeLa cells labelling Ribophorin I (red) with purified ab198508 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198508).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198508).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)
    Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)

    Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab198508 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198508).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)
    Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)

    Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab198508 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198508).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human placenta tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198508).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)
    Anti-Ribophorin I antibody [EPR17043(B)] - BSA and Azide free (ab232599)

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