Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17043(B)] to Ribophorin I - N-terminal
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal
    See all Ribophorin I primary antibodies

  • Description

    Rabbit monoclonal [EPR17043(B)] to Ribophorin I - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: BxPC-3, HepG2, HeLa and PC-3 cell lysates; Human fetal liver lysate. IHC-P: Human placenta and rat liver tissues. ICC/IF: HeLa and MCF7 cells Flow Cyt: HeLa cell

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17043(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
    Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
    All lanes : Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508) at 1/5000 dilution

    Lane 1 : BxPC-3 (Human pancreas adenocarcinoma cells) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 4 : PC-3 (Human prostate cancer cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
    Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

    Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab198508 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow Cytometry - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
    Flow Cytometry - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

    Flow cytometry analysis of HeLa cells labelling Ribophorin I (red) with purified ab198508 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
    Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
    Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508) at 1/2000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human placenta tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
    Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

    Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab198508 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
    Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

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