Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-ICA 512/PTPRN antibody [EPR20718] - BSA and Azide free
See all ICA 512/PTPRN primary antibodies
Description

Rabbit monoclonal [EPR20718] to ICA 512/PTPRN - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Rat
IP	Mouse
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251479 is the carrier-free version of ab207750. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251479 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR20718
Isotype

IgG
Research areas

Western blot - Anti-ICA 512/PTPRN antibody [EPR20718] - BSA and Azide free (ab251479)

All lanes : Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750) at 1/5000 dilution

Lane 1 : Human hippocampus lysate
Lane 2 : Mouse hypothalamus lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse hippocampus lysate
Lane 5 : Rat hippocampus lysate
Lane 6 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 106 kDa
Observed band size: 65 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds

This data was developed using ab207750, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 15596545 and 18824546).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] - BSA and Azide free (ab251479)

This data was developed using ab207750, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human pancreatic islets is observed (PMID: 8641276). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-ICA 512/PTPRN antibody [EPR20718] - BSA and Azide free (ab251479)

This data was developed using ab207750, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized mouse pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive cytoplasmic staining in mouse pancreatic islet cells is observed (PMID: 10820150). The nuclear counter stain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/500 dilution. Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunoprecipitation - Anti-ICA 512/PTPRN antibody [EPR20718] - BSA and Azide free (ab251479)

This data was developed using ab207750, the same antibody clone in a different buffer formulation.

ICA 512/PTPRN was immunoprecipitated from 0.35 mg of mouse hypothalamus tissue lysate with ab207750 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207750 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Mouse hypothalamus lysate 10 µg (Input).

Lane 2: ab207750 IP in mouse hypothalamus tissue lysate (+).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207750 in mouse hypothalamus tissue lysate (-).

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 1 second.
Western blot - Anti-ICA 512/PTPRN antibody [EPR20718] - BSA and Azide free (ab251479)

All lanes : Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750) at 1/1000 dilution

Lane 1 : Rat pancreas tissue lysate
Lane 2 : Human pancreas tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 106 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?

This data was developed using ab207750, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 3 minutes; Lane 2: 5 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID: 15596545; 18824546).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] - BSA and Azide free (ab251479)

This data was developed using ab207750, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on chromaffin cells of mouse adrenal medulla is observed (PMID: 8641276). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] - BSA and Azide free (ab251479)

This data was developed using ab207750, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat pancreatic islets is observed (PMID: 8641276). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-ICA 512/PTPRN antibody [EPR20718] - BSA and Azide free (ab251479)

This data was developed using ab207750, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized rat pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Weak positive cytoplasmic staining in rat pancreatic islet cells is observed (PMID: 10820150). The nuclear counter stain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/500 dilution. Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
Anti-ICA 512/PTPRN antibody [EPR20718] - BSA and Azide free (ab251479)