Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (ab250957)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14000-76] to POC5 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-POC5 antibody [EPR14000-76] - BSA and Azide free
    See all POC5 primary antibodies

  • Description

    Rabbit monoclonal [EPR14000-76] to POC5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250957 is the carrier-free version of ab188330. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250957 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (ab250957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (ab250957)
    This data was developed using ab188330, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling POC5 with ab188330 at 1/50 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (ab250957)
    Western blot - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (ab250957)

    This data was developed using ab188330, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (ab250957)
    Western blot - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (ab250957)
    Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330) at 1/1000 dilution + Y79 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    This data was developed using ab188330, the same antibody clone in a different buffer formulation.

     

  • Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (ab250957)
    Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (ab250957)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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