Anti-SHP2 antibody (ab9214)
Key features and details
- Goat polyclonal to SHP2
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHP2 antibody
See all SHP2 primary antibodies -
Description
Goat polyclonal to SHP2 -
Host species
Goat -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Pig
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Immunogen
Synthetic peptide:
YENVGLMQQQKSFR
, corresponding to C terminal amino acids 580-593 of Human SHP2. -
Positive control
- WB: Human muscle lysate. ICC/IF: HeLa cells Flow Cyt: A431 cells
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General notes
GenBank Accession Number – NP_002825.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SHP2 antibody (ab9214)
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% triton-permeabilized HeLa cells staining SHP2 antibody with ab9214 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. DAPI was used as a nuclear counterstain.
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody at 2ug/ml
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Flow Cytometry - Anti-SHP2 antibody (ab9214)Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton-permeabilized A431 cells (blue) staining SHP2 with ab9214 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 1ug/ml. Primary incubation carried out for one hour.
IgG control: Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody (black).
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Western blot - Anti-SHP2 antibody (ab9214)Anti-SHP2 antibody (ab9214) at 2 µg/ml + Human muscle lysate at 35 µg
Predicted band size: 68 kDa
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Western blot - Anti-SHP2 antibody (ab9214)ab9214 staining (2µ g/ml) of Human Muscle lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab9214 staining (2µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
