Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PTPRH antibody [EPR20897-15] (ab231767)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20897-15] to PTPRH
  • Suitable for: WB, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-PTPRH antibody [EPR20897-15]
    See all PTPRH primary antibodies

  • Description

    Rabbit monoclonal [EPR20897-15] to PTPRH

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-29 and A431 whole cell lysates. ICC: HT-29 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20897-15

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SAP1 antibody [EPR20897-15] (ab231767)
    Western blot - Anti-SAP1 antibody [EPR20897-15] (ab231767)
    All lanes : Anti-PTPRH antibody [EPR20897-15] (ab231767) at 1/1000 dilution

    Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate
    Lane 3 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 122 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: SW480 and Low expression: HCT 116 (PMID: 28713969).

    Exposure time: 37 seconds.

     

  • Immunocytochemistry - Anti-SAP1 antibody [EPR20897-15] (ab231767)
    Immunocytochemistry - Anti-SAP1 antibody [EPR20897-15] (ab231767)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HT-29 cells labelling PTPRH with ab231767 at 1/50 (11.42 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HT-29 cell line. Negative control: SW480 PMID: 28713969). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-SAP1 antibody [EPR20897-15] (ab231767)
    Western blot - Anti-SAP1 antibody [EPR20897-15] (ab231767)
    All lanes : Anti-PTPRH antibody [EPR20897-15] (ab231767) at 1/1000 dilution

    Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HT-29 whole cell lysate treated with PNGase F

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 122 kDa
    Observed band size: 122,250 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 59 seconds.

     

  • Anti-PTPRH antibody [EPR20897-15] (ab231767)
    Anti-PTPRH antibody [EPR20897-15] (ab231767)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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