Anti-SHP2 antibody [Y477] - BSA and Azide free (ab230494)
![Anti-SHP2 antibody [Y477] - BSA and Azide free (ab230494)](https://www.abcam.com/ps/products/230/ab230494/Images/ab230494-311797-anti-shp2-antibody-y477-western-blot.jpg "Anti-SHP2 antibody [Y477] - BSA and Azide free (ab230494)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y477] to SHP2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SHP2 antibody [Y477] - BSA and Azide free
See all SHP2 primary antibodies -
Description
Rabbit monoclonal [Y477] to SHP2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1 cell lysate. A431 and Jurkat cell lysate.
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General notes
Ab230494 is the carrier-free version of ab32159. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab230494 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y477 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SHP2 antibody [Y477] - BSA and Azide free (ab230494)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SHP2 knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 to 4: Merged signal (red and green). Green - ab32159 observed at 68 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32159 was shown to specifically react with SHP2 when SHP2 knockout samples were used. Wild-type and SHP2 knockout samples were subjected to SDS-PAGE. ab32159 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32159).
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![Anti-SHP2 antibody [Y477] - BSA and Azide free (ab230494)](https://www.abcam.com/ps/products/230/ab230494/Images/ab230494-2-benefits-of-recombinant-antibodies.png)
Anti-SHP2 antibody [Y477] - BSA and Azide free (ab230494)
