Anti-SHP2 (phospho Y542) antibody (ab17939)
Key features and details
- Rabbit polyclonal to SHP2 (phospho Y542)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-SHP2 (phospho Y542) antibody
See all SHP2 primary antibodies -
Description
Rabbit polyclonal to SHP2 (phospho Y542) -
Host species
Rabbit -
Specificity
This antibody is specific for SHP2 phosphorylated at tyrosine 542. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic phosphopeptide derived from the region of human SHP2 that contains tyrosine 542. The sequence is conserved in mouse, rat and chicken.
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Positive control
- NIH3T3 cells treated with PDGF.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated SHP2. The final product is generated by affinity chromatography using a SHP2 derived peptide that is phosphorylated at tyrosine 542. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SHP2 (phospho Y542) antibody (ab17939)
Western blot using ab17939 on 10-30
Western blot using ab17939 on 10-30µg NIH3T3 cell lysate.µ g NIH3T3 cell lysate. Lane 1: untreated cells. Lane 2: cells treated with PDGF. Lane 3: cells treated with PDGF. Antibody blocked with non-phosphorylated immunopeptide. Lane 4: cells treated with PDGF. Antibody blocked with a generic tyrosine-phosphorylated peptide. Lane 5: cells treated with PDGF. Antibody blocked with phosphorylated immunopeptide.Lane 1: untreated cells.
Lane 2: cells treated with PDGF.
Lane 3: cells treated with PDGF. Antibody blocked with non-phosphorylated immunopeptide.
Lane 4: cells treated with PDGF. Antibody blocked with a generic tyrosine-phosphorylated peptide.
Lane 5: cells treated with PDGF. Antibody blocked with phosphorylated immunopeptide.
