Anti-SHP2 antibody [M163] (ab76285)
![Anti-SHP2 antibody [M163] (ab76285)](https://www.abcam.com/ps/products/76/ab76285/Images/ab76285-259118-anti-shp2-antibody-m163-western-blot.jpg "Anti-SHP2 antibody [M163] (ab76285)")
Key features and details
- Mouse monoclonal [M163] to SHP2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-SHP2 antibody [M163]
See all SHP2 primary antibodies -
Description
Mouse monoclonal [M163] to SHP2 -
Host species
Mouse -
Specificity
ab76285 does not cross-react with SHP1. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Recombinant fragment corresponding to Human SHP2 (N terminal).
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Positive control
- Human A431 and Jurkat cells, and adult mouse brain lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M163 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-SHP2 antibody [M163] (ab76285)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SHP2 knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 to 4: Merged signal (red and green). Green - ab76285 observed at 68 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76285 was shown to specifically react with SHP2 when SHP2 knockout samples were used. Wild-type and SHP2 knockout samples were subjected to SDS-PAGE. ab76285 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-SHP2 antibody [M163] (ab76285)](https://www.abcam.com/ps/products/76/ab76285/Images/ab76285-91645-anti-shp2-antibody-m163-western-blot.gif)
Western blot - Anti-SHP2 antibody [M163] (ab76285)Lane 1 : Anti-SHP2 antibody [M163] (ab76285) at 1/250 dilution
Lane 2 : Anti-SHP2 antibody [M163] (ab76285) at 1/500 dilution
Lane 3 : Anti-SHP2 antibody [M163] (ab76285) at 1/1000 dilution
Lane 4 : Anti-SHP2 antibody [M163] (ab76285) at 1/2000 dilution
All lanes : adult mouse brain lysates
Predicted band size: 68 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
