Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD11b antibody [EPR19387] - BSA and Azide free (ab232427)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19387] to CD11b - BSA and Azide free
  • Suitable for: ICC/IF, IP, Flow Cyt, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD11b antibody [EPR19387] - BSA and Azide free
    See all CD11b primary antibodies

  • Description

    Rabbit monoclonal [EPR19387] to CD11b - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: RAW 264.7 cells.

  • General notes

    Ab232427 is the carrier-free version of ab184308. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232427 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Flow Cytometry - Anti-CD11b antibody [EPR19387] - BSA and Azide free (ab232427)
    Flow Cytometry - Anti-CD11b antibody [EPR19387] - BSA and Azide free (ab232427)

    Flow cytometric analysis of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling CD11b with ab184308 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] -Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184308).

  • Immunoprecipitation - Anti-CD11b antibody [EPR19387] - BSA and Azide free (ab232427)
    Immunoprecipitation - Anti-CD11b antibody [EPR19387] - BSA and Azide free (ab232427)

    CD11b was immunoprecipitated from 0.35 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab184308 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184308 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).

    Lane 2: ab184308 IP in RAW 264.7 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab184308 in RAW 264.7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184308).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] - BSA and Azide free (ab232427)
    Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] - BSA and Azide free (ab232427)

    Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) and WEHI-231 (Mouse B cell lymphoma cell line) cells labeling CD11b with ab184308 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green).

    Confocal image showing membrane staining on RAW 264.7 cells and no staining on WEHI-231 cells (negative cell line).

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1,000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1,000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab184308 at 1/500 dilution followed by ab150120  at 1/1,000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1,000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184308).

  • Anti-CD11b antibody [EPR19387] - BSA and Azide free (ab232427)
    Anti-CD11b antibody [EPR19387] - BSA and Azide free (ab232427)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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