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Anti-Integrin alpha V+beta 5 antibody [P1F6] - BSA and Azide free (ab223605)

Key features and details

  • Mouse monoclonal [P1F6] to Integrin alpha V+beta 5 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Integrin alpha V+beta 5 antibody [P1F6] - BSA and Azide free
    See all Integrin alpha V+beta 5 primary antibodies

  • Description

    Mouse monoclonal [P1F6] to Integrin alpha V+beta 5 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. Small cell lung carcinoma cells (UCLA P3).

  • Positive control

    • This antibody gave a positive signal in IF and Flow Cytometry in A549 cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] - BSA and Azide free (ab223605)
    Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] - BSA and Azide free (ab223605)

    This data was developed using the same antibody clone in a different buffer formulation (ab223605)

    Flow Cytometry Overlay histogram showing A549 cells stained with ab223605 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab223605) (1x106 cells in 100µl at 1µg/ml) for 30 min on ice.

    The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min on ice.

    Isotype control antibody (black line) was mouse IgG1κ; (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

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