Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20841] to GSDMB - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GSDMB antibody [EPR20841] - BSA and Azide free
    See all GSDMB primary antibodies

  • Description

    Rabbit monoclonal [EPR20841] to GSDMB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein within Human GSDMB. The exact sequence is proprietary.
    Database link: Q8TAX9

  • Positive control

    • Flow Cyt: A431 cells.

  • General notes

    Ab226483 is the carrier-free version of ab215729. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226483 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20841

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)
    Immunoprecipitation - Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)

    GSDMB was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DDDDK tagged GSDMB expression vector, whole cell lysate with ab215729 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215729 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate 10 µg (Input). 

    Lane 2: ab215729 IP in HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215729 in HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215729).

  • Immunoprecipitation - Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)
    Immunoprecipitation - Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)

    GSDMB was immunoprecipitated from 0.35 mg of A431 (human epidermoid carcinoma cell line) whole cell lysate with ab215729 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215729 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: A431 whole cell lysate 10 µg (Input). 

    Lane 2: ab215729 IP in A431 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215729 in A431 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215729).

  • Flow Cytometry - Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)
    Flow Cytometry - Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized A431 (human epidermoid carcinoma cell line) cell line labeling GSDMB with ab215729 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215729).

  • Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)
    Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)

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