Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)
![Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)](https://www.abcam.com/ps/products/226/ab226483/Images/ab226483-321347-anti-gsdmb-antibody-epr20841-immunoprecipitation.jpg "Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20841] to GSDMB - BSA and Azide free
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-GSDMB antibody [EPR20841] - BSA and Azide free
See all GSDMB primary antibodies -
Description
Rabbit monoclonal [EPR20841] to GSDMB - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human GSDMB. The exact sequence is proprietary.
Database link: Q8TAX9 -
Positive control
- Flow Cyt: A431 cells.
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General notes
Ab226483 is the carrier-free version of ab215729. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab226483 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20841 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)
GSDMB was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DDDDK tagged GSDMB expression vector, whole cell lysate with ab215729 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215729 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate 10 µg (Input).
Lane 2: ab215729 IP in HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215729 in HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215729).
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![Immunoprecipitation - Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)](https://www.abcam.com/ps/products/226/ab226483/Images/ab226483-321346-anti-gsdmb-antibody-epr20841-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)GSDMB was immunoprecipitated from 0.35 mg of A431 (human epidermoid carcinoma cell line) whole cell lysate with ab215729 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215729 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: A431 whole cell lysate 10 µg (Input).
Lane 2: ab215729 IP in A431 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215729 in A431 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215729).
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![Flow Cytometry - Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)](https://www.abcam.com/ps/products/226/ab226483/Images/ab226483-287529-anti-gsdmb-antibody-epr20841-flow-cytometry.jpg)
Flow Cytometry - Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized A431 (human epidermoid carcinoma cell line) cell line labeling GSDMB with ab215729 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215729).
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![Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)](https://www.abcam.com/ps/products/226/ab226483/Images/ab226483-4-benefits-of-recombinant-antibodies.png)
Anti-GSDMB antibody [EPR20841] - BSA and Azide free (ab226483)
