Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GSDMB antibody [EPR20841] (ab215729)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20841] to GSDMB
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GSDMB antibody [EPR20841]
    See all GSDMB primary antibodies

  • Description

    Rabbit monoclonal [EPR20841] to GSDMB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein within Human GSDMB. The exact sequence is proprietary.
    Database link: Q8TAX9

  • Positive control

    • WB: HEK-293T transfected with human DDDDK tagged GSDMB expression vector whole cell lysate; A431 whole cell lysate. Flow Cyt: A431 cells. IP: HEK-293T transfected with DDDDK tagged GSDMB expression vector whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20841

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GSDMB antibody [EPR20841] (ab215729)
    Western blot - Anti-GSDMB antibody [EPR20841] (ab215729)
    All lanes : Anti-GSDMB antibody [EPR20841] (ab215729) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), transfected with an empty vector (vector control), containing a DDDDK tag, whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), transfected with human DDDDK tagged GSDMB expression vector, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 10 seconds
  • Western blot - Anti-GSDMB antibody [EPR20841] (ab215729)
    Western blot - Anti-GSDMB antibody [EPR20841] (ab215729)
    Anti-GSDMB antibody [EPR20841] (ab215729) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-GSDMB antibody [EPR20841] (ab215729)
    Flow Cytometry - Anti-GSDMB antibody [EPR20841] (ab215729)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized A431 (human epidermoid carcinoma cell line) cell line labeling GSDMB with ab215729 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-GSDMB antibody [EPR20841] (ab215729)
    Immunoprecipitation - Anti-GSDMB antibody [EPR20841] (ab215729)

    GSDMB was immunoprecipitated from 0.35 mg of A431 (human epidermoid carcinoma cell line) whole cell lysate with ab215729 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215729 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution

    Lane 1: A431 whole cell lysate 10 µg (Input). 

    Lane 2: ab215729 IP in A431 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215729 in A431 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-GSDMB antibody [EPR20841] (ab215729)
    Immunoprecipitation - Anti-GSDMB antibody [EPR20841] (ab215729)

    GSDMB was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DDDDK tagged GSDMB expression vector, whole cell lysate with ab215729 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215729 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate 10 µg (Input). 

    Lane 2: ab215729 IP in HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215729 in HEK-293T transfected with DDDDK tagged GSDMB expression vector, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-GSDMB antibody [EPR20841] (ab215729)
    Anti-GSDMB antibody [EPR20841] (ab215729)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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