Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GBP1 antibody [1B1] (ab252832)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [1B1] to GBP1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GBP1 antibody [1B1]
    See all GBP1 primary antibodies

  • Description

    Rat monoclonal [1B1] to GBP1

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • WB: HeLa treated with 100 U/ml IFN gamma (ab9659) for 24 hours, whole cell lysate. IHC-P: Human spleen and tonsil tissue. ICC/IF: HeLa treated with IFN gamma (100 U/ml 24h).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-GBP1 antibody [1B1] (ab252832)
    Western blot - Anti-GBP1 antibody [1B1] (ab252832)
    All lanes : Anti-GBP1 antibody [1B1] (ab252832) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa treated with 100U/ml IFN gamma (ab9659) for 24 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 68 kDa
    Observed band size: 67 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    The expression profile observed is consistent with what has been described in the literature (PMID:19079332)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [1B1] (ab252832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [1B1] (ab252832)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for GBP1 using ab252832 at 1/100 dilution in immunohistochemical analysis followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Cytoplasmic staining on human spleen. The section was incubated with ab252832 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. 

    Secondary antibody only control: Used PBS instead of primary antibody. Secondary antibody was a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [1B1] (ab252832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [1B1] (ab252832)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for GBP1 using ab252832 at 1/100 dilution in immunohistochemical analysis followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Cytoplasmic staining on human tonsil. The section was incubated with ab252832 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. 

    Secondary antibody only control: Used PBS instead of primary antibody. Secondary antibody was a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

  • Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [1B1] (ab252832)
    Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [1B1] (ab252832)

    ICC/IF analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (+/-IFN gamma (100U/ml 24h)) cells labeling GBP1 (Green) using ab252832 at 1/30 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Tubulin was counterstained using ab179504  Anti-beta IV Tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 diluton (Red). The nuclear counterstain was DAPI (Blue). Confocal image showing cytoplasmic staining in HeLa cells treated with interferon gamma (100 U/ml) for 24 hours.

  • Anti-GBP1 antibody [1B1] (ab252832)
    Anti-GBP1 antibody [1B1] (ab252832)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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