Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)
![Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)](https://www.abcam.com/ps/products/29/ab29045/Images/ab29045-210573-anti-stat1-phospho-y701-antibody-m135-western-blot.jpg "Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)")
Key features and details
- Mouse monoclonal [M135] to STAT1 (phospho Y701)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-STAT1 (phospho Y701) antibody [M135]
See all STAT1 primary antibodies -
Description
Mouse monoclonal [M135] to STAT1 (phospho Y701) -
Host species
Mouse -
Specificity
The antibody detects 84 and 91 kDa stat1 variants on SDS-PAGE immunoblots of human A431 treated with EGF, as well as Jurkat and A431 cells treated with pervanadate. The antibody does not detect these variants in control cells. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide corresponding to Human STAT1 (phospho Y701) conjugated to Keyhole Limpet Haemocyanin (KLH).
-
Positive control
- human SKOV-3 and MDA-MB-468 cells, ab7909, Jurkat cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Mouse monoclonal antibody purified with protein A chromatography. -
Clonality
Monoclonal -
Clone number
M135 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)Lanes 1-2 & 4 : Anti-STAT1 antibody (ab2415)
Lane 3 : Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)
Lanes 5-6 : Anti-STAT3 antibody (ab5073)
Lanes 7-8 : Anti-STAT5 antibody (ab68465)
Lanes 9-10 : anti-Stat5 (Tyr-694)
All lanes :A-431 whole cell lysate (ab7909)
Predicted band size: 87 kDaWestern blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10).
-
![Western blot - Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)](https://www.abcam.com/ps/products/29/ab29045/Images/ab29045-40194-anti-stat1-phospho-y701-antibody-m135-western-blot.jpg)
Western blot - Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)Lanes 1-2 : Anti-Stat1
Lanes 3-4 : Anti-STAT1 (phospho Y701) antibody [M135] (ab29045) at 1/1000 dilution
Lanes 1 & 3 : Jurkat cells
Lanes 2 & 4 : Jurkat cells treated with 1mM pervanadate
Predicted band size: 87 kDa
Observed band size: 84,91 kDa why is the actual band size different from the predicted?
