Anti-STAT1 antibody (ab47425)
Key features and details
- Rabbit polyclonal to STAT1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAT1 antibody
See all STAT1 primary antibodies -
Description
Rabbit polyclonal to STAT1 -
Host species
Rabbit -
Specificity
ab47425 detects endogenous levels of total STAT1 protein. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human STAT1 aa 694-743.
Database link: P42224 -
Positive control
- WB: 293 cells. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-STAT1 antibody (ab47425)Anti-STAT1 antibody (ab47425) at 1/2000 dilution + 293 Cells
Predicted band size: 87 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody (ab47425)ab47425 staining STAT1 in human breast carcinoma tissue by Immunohistochemistry (IHC-P- paraformaldehyde fixed , paraffin embedded sections). Samples were incubated with primary antibody at a 1:50 dilution.
Right picture: Blocked with synthesized peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody (ab47425)ICC/IF image of ab47425 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47425, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
