Anti-STAT5a (phospho S780) antibody (ab30649)
Key features and details
- Rabbit polyclonal to STAT5a (phospho S780)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAT5a (phospho S780) antibody
See all STAT5a primary antibodies -
Description
Rabbit polyclonal to STAT5a (phospho S780) -
Host species
Rabbit -
Specificity
ab30649 detects endogenous levels of STAT5A only when phosphorylated at Serine 780. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human STAT5a. Synthesized phosphopeptide derived from human STAT5A around the phosphorylation site of Serine 780.
Database link: P42229 -
Positive control
- IHC: human breast carcinoma. WB: Jurkat treated with 125 ng/mL PMA for 30 minutes lysate. 293T treated with 125 ng/mL PMA for 30 minutes lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab30649 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-STAT5a (phospho S780) antibody (ab30649)All lanes : Anti-STAT5a (phospho S780) antibody (ab30649) at 1/1000 dilution
Lane 1 : Jurkat untreated lysate
Lane 2 : Jurkat treated with 125 ng/mL PMA for 30 minutes lysate
Lane 3 : 293T untreated lysate
Lane 4 : 293T treated with 125 ng/mL PMA for 30 minutes lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 91 kDaLoading control: beta Actin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a (phospho S780) antibody (ab30649)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ab30649. The picture on the right is blocked with the phospho peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-STAT5a (phospho S780) antibody (ab30649)ICC/IF image of ab30649 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30649, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-STAT5a (phospho S780) antibody (ab30649)All lanes : Anti-STAT5a (phospho S780) antibody (ab30649)
Lane 1 : HeLa cells extract
Lane 2 : HeLa cells extract with synthesized non-phosphopeptide
Lane 3 : HeLa cells extract with synthesized phosphopeptide
Predicted band size: 91 kDa
Observed band size: 91 kDa
