Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1914(2)] to STAT5a (phospho S726)
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-STAT5a (phospho S726) antibody [EPR1914(2)]
See all STAT5a primary antibodies -
Description
Rabbit monoclonal [EPR1914(2)] to STAT5a (phospho S726) -
Host species
Rabbit -
Specificity
ab128896 only detects STAT5a phosphorylated at Serine 726 -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Phospho specific peptide corresponding to residues surrounding Ser726 of Human STAT5a (UniProt P42229).
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Positive control
- SH-SY5Y cell lysates treated with Okadaic acid and Calyculin A.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1914(2) -
Isotype
IgG -
Research areas
Images
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Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking & dilution buffer: 5% NFDM/TBST
Lane 1 sample: STAT5a (pS726) phospho peptide
Lane 2 sample: STAT5a non-phospho peptide
Exposure time: 10 seconds
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All lanes : Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896) at 1/5000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma) treated with Okadaic acid and Calyculin A whole cell lysates
Lane 2 : Untreated SH-SY5Y (human neuroblastoma) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST
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All lanes : Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysates: untreated
Lane 2 : SH-SY5Y cell lysates: treated with Okadaic acid and Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
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