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Signal Transduction Signaling Pathway Nuclear Signaling STATs

Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)

Price and availability

308 236 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1914(2)] to STAT5a (phospho S726)
  • Suitable for: Dot blot, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-STAT5a (phospho S726) antibody [EPR1914(2)]
    See all STAT5a primary antibodies
  • Description

    Rabbit monoclonal [EPR1914(2)] to STAT5a (phospho S726)
  • Host species

    Rabbit
  • Specificity

    ab128896 only detects STAT5a phosphorylated at Serine 726
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Phospho specific peptide corresponding to residues surrounding Ser726 of Human STAT5a (UniProt P42229).

  • Positive control

    • SH-SY5Y cell lysates treated with Okadaic acid and Calyculin A.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1914(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Images

  • Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
    Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)

    Primary antibody dilution: 1/1000

    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

    Secondary antibody dilution: 1/2500

    Blocking & dilution buffer: 5% NFDM/TBST

    Lane 1 sample: STAT5a (pS726) phospho peptide

    Lane 2 sample: STAT5a non-phospho peptide

    Exposure time: 10 seconds

  • Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
    Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
    All lanes : Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896) at 1/5000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma) treated with Okadaic acid and Calyculin A whole cell lysates
    Lane 2 : Untreated SH-SY5Y (human neuroblastoma) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
    Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
    All lanes : Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysates: untreated
    Lane 2 : SH-SY5Y cell lysates: treated with Okadaic acid and Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa

  • Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
    Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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