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Signal Transduction Signaling Pathway Nuclear Signaling STATs

Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095)

Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [6.F1] to STAT5a (phospho Y694)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-STAT5a (phospho Y694) antibody [6.F1]
    See all STAT5a primary antibodies
  • Description

    Mouse monoclonal [6.F1] to STAT5a (phospho Y694)
  • Host species

    Mouse
  • Specificity

    ab106095 is specific for STAT5a protein phosphorylated at Y694.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    A synthetic peptide corresponding to residues surrounding Y694 of Mouse STAT5a protein.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    NK92 cell lysate; Human breast tissue; 3T3 cells.
  • Clonality

    Monoclonal
  • Clone number

    6.F1
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Images

  • Western blot - Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095)
    Western blot - Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095)
    All lanes : Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095) at 1/1000 dilution (overnight at 4°C)

    Lane 1 : NK92 cell lysate, untreated
    Lane 2 : NK92 cell lysate, treated with 1Ku of IL2 (30min)

    Secondary
    All lanes : Gt-a-Mouse IgG DyLight™800 conjugated at 1/20000 dilution

    Predicted band size: 91 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095)
    ab106095 at 20&microg/ml staining STAT5a in Formalin-fixed, Paraffin-embedded Human breast tissue by Immunohistochemistry.
    The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095)
    ab106095 staining STAT5a in 3T3 cells by Immunofluorescence (red). Green staining represents tubulin.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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